Weiblen, George D , Craft, KJ , Wenger, Jonathan P , Mehmedic, Z. , ElSohly, M. .
A genetic linkage map for Cannabis based on microsatellites and AFLP markers.
We screened an inbred population of Cannabis sativa using microsatellite and AFLP markers for the purpose of mapping quantitative trait loci (QTL) in this economically important species. Dioecious hemp and marijuana cultivars were subject to five generations of full-sib inbreeding and crossed to produce an F1 mapping population. A single F1 was selfed using silver nitrate to induce fertile male flowers in a genetically female plant. F2 plants genotyped for microsatellite and AFLP markers were used to construct a linkage map with JoinMap software. Linkage groups segregating with drug content and other economically important traits were identified. Screening of the F2 population using single nucleotide polymorphisms (SNPs) from an expressed sequence tag (EST) library of hemp and marijuana line is proposed to better resolve major QTL.
Log in to add this item to your schedule
1 - University of Minnesota, Department of Plant Biology, 250 Biological Sciences, 1445 Gortner Avenue, St. Paul, Minnesota, 55108, USA
2 - University of Minnesota, Plant Biology, 250 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN, 55108, U.S.A.
3 - University of Minnesota, Department of Plant Biology, 250 Biological Sciences Center, 1445 Gortner Avenue, Saint Paul, MN, 55108, USA
4 - University of Mississippi
Presentation Type: Oral Paper:Papers for BSA Sections
Location: Cottonwood C/Snowbird Center
Date: Monday, July 27th, 2009
Time: 11:30 AM